ABSTRACT This report examines the effects of calcium anacardate (CA) on the morphology, histology, and proteomics of the testes and epididymal ducts of breeding quail. To that end, 22 140-day-old males were grouped in four treatments, each with different concentrations of CA: 0% (control), 0.25%, 0.50%, and 0.75%. The testicles of the animals were weighed and measured, and proteins from the testicles were separated by 1D SDS-PAGE and analyzed via mass spectrometry. Overall, 35 bands were identified in the testicular tissue gels of European quail. Of these, 13 were significantly different, with treatment 0.75% CA being different from the control in all bands. Significant differences among the mean heights of the seminiferous epithelium and diameters of the epididymal duct were observed. In the control group, the average height of the seminiferous epithelium was 71.2 µm, and in 0.75% CA, it was 45.6 µm. Supplementing feed with CA altered the expression of specific proteins, such as superoxide dismutase and heat shock proteins, of the seminal plasma of quail, in addition to facilitating histological alterations regarding the height of the seminiferous epithelium and the diameter of the epididymal duct, improving the reproductive parameters.

RESUMO: Pesquisas têm sido realizadas para identificar as proteínas presentes no plasma seminal canino, com o intuito de relacioná-las com a qualidade espermática, bem como buscar por marcadores moleculares de patologias do trato reprodutivo. Há evidências de que as proteínas ligadoras de heparina, ligadoras de zinco, a lactoferrina, bem como as enzimas matrix metalloproteinase, superoxide dismutase, catalase e a glutationa peroxidase estão relacionadas com a qualidade seminal canina. Outras pesquisas indicam que a prolactina, e as enzimas arginina esterase, fosfatase ácida e fosfatase alcalina poderiam ser utilizadas com sucesso como biomarcadores de doenças reprodutivas. Assim, esta revisão de literatura objetiva abordar aspectos relacionados às proteínas do plasma seminal canino, suas influências sobre a fertilidade, e sua importância como biomarcadores de doenças reprodutivas.

ABSTRACT: Studies have been performed to identify the proteins present in canine seminal plasma (SP) and relate them to sperm quality as well as to discover molecular markers of reproductive tract diseases. There is evidence that heparin-binding proteins, zinc-binding proteins, and lactoferrin as well as the matrix metalloproteinase, superoxide dismutase, catalase, and glutathione peroxidase enzymes are associated with canine sperm quality. Other studies indicate that prolactin and enzymes like arginine esterase, acid phosphatase, and alkaline phosphatase could be successfully used as biomarkers of reproductive disorders. Thus, the present literature review aims to address aspects related to proteins of the canine SP, their influence on fertility, and their importance as biomarkers of reproductive disorders.

O presente estudo foi conduzido objetivando-se avaliar a influência do estresse climático sobre o desempenho produtivo e as respostas fisiológicas de ovinos da raça Santa Inês em confinamento. Foram avaliados dois ambientes (sombra e sol) e duas dietas com duas relações volumoso:concentrado (70C:30V: 70% de concentrado e 30% de volumoso; 30C:70V: 30% de concentrado e 70% de volumoso). Os animais mantidos à sombra e alimentados com dieta contendo alto teor de concentrado (70C:30V) apresentaram maior consumo de matéria seca (1258 g/animal/dia) e de proteína bruta (0,8% do peso vivo [PV] e 18 g/PV0,75) e maior ganho de peso (247 g/animal/dia). A temperatura nos ambientes de sol e sombra durante a tarde foi de 32,1 e 30,6:C e os valores do índice de temperatura e umidade (ITU), 82,3 e 81,1, respectivamente. Os animais alimentados com alto teor de concentrado (70C:30V) apresentaram maior freqüência respiratória (FR), tanto à sombra quanto ao sol (87 e 71 mov/min, respectivamente). Durante a tarde, a temperatura retal (TR) dos animais foi maior (39,1:C) que pela manhã (38,9:C). Porém, a TR mais elevada (39,3:C) foi observada nos animais mantidos à sombra e alimentados com alto percentual de concentrado (70C:30V). Independentemente do ambiente, os animais alimentados com alto teor de concentrado (70C:30V) apresentaram maior TR (39,2:C) do que aqueles alimentados com reduzido teor de concentrado (30C:70V) (38,8:C). O tipo de dieta teve efeito sobre a susceptibilidade dos animais ao estresse causado pelas condições ambientais críticas durante o experimento. Os animais da raça Santa Inês mostraram-se sensíveis ao estresse ambiental, uma vez que apresentaram menor desempenho produtivo, quando expostos a condições de ausência de sombra.

A study was conducted to determine the effect of environmental stress on physiological criteria of feedlot sheep. Treatments consisted of two different housing conditions (shade and no shade) and two levels of concentrate in the diet: high concentrate (70% of concentrate and 30% of roughage-70C:30R); low concentrate (30% of concentrate and 70% of roughage-30C:70R). Animals raised under shade and fed a high concentrate diet (70C:30R) had greater dry mater intake (1258 g/animal/day) and crude protein (0.8% of body weight (BW) and 18 g/BW0.75), as well as weight gain (247 g/animal/day). Air temperature in the afternoon was 30.6:C, under shade, and 32.1:C, under sun and values of temperature humidity index(THI), 82.3 and 81.1, respectively. Animals fed more concentrate (70C:30R) had also higher respiratory frequency (RF), either raised under shade (87 mov/min) or under the sun (71 mov/min.). Rectal temperature (RT) was higher in the afternoon (39.1:C) than in the morning (38.9:C), but animals raised under shade and fed diets with 70% concentrate (70C:30R) had the highest value of RT (39.3:C). Regardless of type of housing conditions, the animals that received more concentrate (70C:30R) had greater rectal temperature (39.2:C) than those fed a diet with less concentrate (30C:70R) (38.8:C). Therefore, the type of diet influenced the response of the animals to housing conditions. Moreover, Santa Inês sheep are susceptible to environmental stress because had lower performance when raised under no shade.

O trabalho foi conduzido com o objetivo de avaliar a utilização de concentrados contendo farelo de castanha de caju (FCC) na alimentação de ovinos mantidos em confinamento. Foram testados quatro tipos de concentrados isoprotéicos contendo 0, 12, 24 e 36% de FCC, tendo o concentrado participado com 30% da matéria seca (MS) da dieta total. Foram utilizados 32 ovinos sem raça definida (SRD), machos e fêmeas, em esquema fatorial 4 x 2 (quatro níveis de inclusão de FCC e dois sexos) e quatro repetições. Foram avaliados ganho de peso e conversão alimentar, bem como os consumos de matéria seca (CMS), proteína bruta (CPB), extrato etéreo (CEE) e fibra em detergente neutro (CFDN). Estas variáveis foram analisadas conforme o consumo em g/animal/dia, % do peso vivo (PV) e g/UTM (PV0,75), tendo sido feita a análise de variância com teste de médias e estudo de regressão. Foram observadas diferenças no CMS, CPB e CEE de animais alimentados com dietas com 0 e 36% de FCC, com tendência de diminuição dos consumos de MS, PB e FDN, à medida que se aumentou o FCC na dieta. O consumo de EE elevou-se com a adição FCC às dietas. O ganho de peso e a conversão alimentar foram semelhantes para as quatro dietas fornecidas. Portanto, o FCC deve ser utilizado com um nível de inclusão de 24% do concentrado, não ultrapassando 6% de lipídios na dieta total.

A study was conducted to evaluate the use of cashew nuts meal (CNM) in diets for sheep, raised in feedlot. Thirty two crossbred animals received concentrates with the same amount of crude protein, but four different levels of CNM (0, 12, 24 and 36%). A factorial 4 x 2 was used, with four levels of CNM and 2 sexes, with four repetitions. Daily weight gain and feed conversion as well as intake of dry matter (DM), crude protein (CP), ether extract (EE), neutral detergent fiber(NDF) and ashes were determined by analysis of variance, Duncan statistical test and regression analysis. Intake was estimated as g/animal/day, percentage of body weight (%BW) and g/BW0.75. There were differences in intake of DM, CP and EE when animals were fed concentrates with 0 and 36% of CNM. Also, intake of DM, CP and NDF tended to decrease as the levels of CNM increased in the diet. EE intake increased according to the different CNM levels. The type of diet did not significantly affect either weight gain or feed conversion. Therefore, cashew nut meal can be included up to 24% in the concentrate, but the total diets should not have more than 6% of fat.